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CBSE Class 12 Biotechnology Syllabus 2024-25 (Latest) PDF Download

CBSE (Central Board of Secondary Education) has released the CBSE Class 12 Biotechnology Syllabus 2024-25 for next year’s board exam. Students of class 12 who are preparing for the academic session 2024-25. They should download this latest CBSE Class 12th Biotechnology Syllabus and read every topic mentioned in the latest syllabus.

CBSE Class 12 Students who are preparing for the CBSE Board Exam 2025 can download the latest CBSE Class 12th Biotechnology syllabus 2024-25 through the direct link given in the article or the official website of CBSE at Class 12 aspirants read the latest CBSE Biotechnology Syllabus 2024-25 Class 12 given below carefully and prepare according to the same syllabus for Board Exam 2024.

CBSE Board has released the latest syllabus of Biotechnology for all class 12 students. According to this latest syllabus, all the class 12 students have to prepare for the academic session 2024-25. The link to download the PDF of this Biotechnology Syllabus is given in this article from where you can download the PDF of this subject.

Importance of CBSE Class 12 Biotechnology Syllabus 2024-25

CBSE Class 12 Biotechnology Syllabus 2024-25: Equally significant developments in technology along with the unprecedented growth of human knowledge in the field of biological sciences have brought about significant changes in the existing social and economic systems. The emerging field of biotechnology is expected to further enhance the applications of science and technology for human welfare. Modern biotechnological processes include a wide range of new products such as antibiotics, vaccines, monoclonal antibodies, and many others. In addition, the development of recombinant DNA technology has yielded many new useful products in the fields of healthcare and agriculture.

The present curriculum takes care of all these aspects. There is a full emphasis on introducing the learners to the fundamental concepts, basic techniques, and their applications. It is expected that the knowledge gained through the study of various subjects and the skills acquired through prescribed practical work will enable the learners to face the challenges of academic as well as vocational courses after studying the subject at the senior secondary level.

CBSE Biotechnology Syllabus 2024-25 for Class 12

The theory exam of Biotechnology will be 70 Marks and the practical exam will be 30 marks. Class 12 students will get 3 hours for the theory exam of Biotechnology and 3 hours for the practical exam also.

CBSE Class 12th Biotechnology Syllabus 2024-25 Course Structure

UnitsUnits NameMarks
VProtein and Gene Manipulation40
VICell Culture and Genetic Manipulation30

Unit-V Protein and Gene Manipulation 40 Marks

Chapter 1: Recombinant DNA Technology

Introduction, Tool of Recombinant DNA technology, Making rDNA molecule, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Reaction (PCR), DNA Sequencing.

Chapter 2: Protein Structure and Engineering

Introduction to the world of proteins, Structure-function Relationship in proteins, Characterization of proteins, Protein products, Designing proteins (Protein Engineering)

Chapter 3: Genomics, Proteomics, and Bioinformatics

Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Functional genomics, Proteomics, Information sources, and Analysis using bioinformatics tools.

Unit-VI Cell Culture and Genetic Manipulation (30 Marks)

Chapter 1: Microbial Cell Culture and its Applications

Introduction, Microbial nutrition and culture techniques, Measurement and kinetics of microbial growth, Isolation of microbial products, Strain isolation and improvement, Applications of microbial culture technology.

Chapter 2: Plant Cell Culture and Applications

Introduction, Cell, and tissue culture techniques, Applications of cell and tissue culture, Transgenic plants with beneficial traits, Biosafety of transgenic plants

Chapter 3: Animal Cell Culture and Applications

Introduction, Animal cell culture techniques, Applications of animal cell culture, Stem cell technology.


Note: Every student will be required to do the following experiments during the academic session.

  1. Use of special equipment in biotechnology experiments
  2. Isolation of bacterial plasmid DNA
  3. Detection of DNA by gel electrophoresis
  4. Estimation of DNA by UV spectroscopy
  5. Isolation of bacteria from curd & staining of bacteria
  6. Cell viability assay using Evan’s blue dye exclusion method
  7. Data retrieval and database search using the internet site NCBI and a DNA and protein sequence from the internet, analyze it, and comment on it.
  8. Reading a DNA sequencing gel to arrive at the sequence
  9. Project work

The Scheme of Evaluation for Practical Marks 2024-25

ATwo Experiments6+6 (only one computer-based practical)
Practical Record4
Viva on Practical4
BProject Work
Write up5
Viva on project5

Prescribed Books

  • A Text-Book of Biotechnology – Class XII: Published by CBSE, New Delhi
  • A Laboratory Manual of Biotechnology – Class XII: Published by CBSE, New Delhi

CBSE Class 12 Biotechnology Syllabus 2024-25 PDF: Download

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My Name is Mukesh Kumar. I am a Teacher, Blogger, Educational Content Writer, and Founder of CBSE Digital Education.

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